1.2.2. Remove the growth media and wash the sample with balanced salt solution to remove any remaining growth media or other contaminants. For example, we use ice-cold 1× PBS for mammalian cell culture and 1× M9 buffer for C. elegans; 1.2.3. After washing, add a minimal amount of ice-cold salt solution to the sample.
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عرض المزيدFor more than 20 years, we have been specializing in the development, manufacture, marketing and after-sales service of grinding media. We are a professional supplier of grinding media, including Dia.25mm-150mm(1''-6'') forged and rolling grinding steel balls, Dia.20-150mm chrome alloyed casting iron balls, grinding rod and grinding cylpebs.
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عرض المزيدCell Separation Sciencedirect. Cell Separation Methods and Selected Applications Volume 4 provides information pertinent to the design and application of methods for the separation of cells This book covers a variety of topics including liver cells epidermal Langerhans cells isolation of oval cells clonal analysis and the purification of polymorphonuclear leukocytes
عرض المزيدThe present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein …
عرض المزيدFig. S1: Figure S1.Protein expression stoichiometry in multi-enzyme pathways is conserved across growth conditions and additional species, related to Fig. 1 (A) Details for the metabolic pathways and corresponding enzymes shown in Fig. 1C from the main text (enzymes with sequence homology below our threshold in gray).Gene names are for E. coli.(B-F) …
عرض المزيدDynabeads products, types, and uses Dynabeads products come in several sizes and with different surface functionalities, for use in a wide variety of applications. Some beads are pre-coupled with a biomolecule (ligand) that can be an antibody, protein or antigen, DNA/RNA probe, or any other molecule with an affinity for the desired target.
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عرض المزيدDNA-Dynabeads Affinity Purification of Proteins for Mass Spectrometry Analysis---Yeast YPH499 wild-type cells were grown in 2 liters YPG media at 30°C to OD600 near 1.5. Cells were harvested by centrifugation at 2600x g for 20 minutes and washed once with ice-cold water.
عرض المزيدInvitrogen RNAlater Stabilization Solution is an aqueous, nontoxic tissue RNA stabilization and storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater solution minimizes the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater …
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عرض المزيدGrinding media is used to finely grind material to a size which allows recovery of the desired contained material. It comes in different forms such as grinding balls, grinding cylpebs, rods or ...
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عرض المزيدGFP-tags and cryogenic grinding with cell lines that express the protein of interest at near endogenous levels. This method provides for high yield, low background affinity isolations using only modest quantities of cell material.
عرض المزيدAuthor Summary Chloroplasts are subcellular organelles in plants and algae that carry out the core reactions of photosynthesis. Chloroplasts originated as cyanobacterial endosymbionts. Subsequent coevolution with their eukaryotic host resulted in a massive transfer of genes to the nuclear genome, the acquisition of new gene expression mechanisms, and the …
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عرض المزيدThe tissue suspension was further ground with 5-mm steel grinding balls at 50 cycles/s for 5 min in a tissue homogenizer (Qiagen TissueLyser LT) at 4°C. Homogenized samples were then heated at 95°C for 5 min, briefly vortexed, and allowed to rest on ice for 5 min. Samples were then centrifuged at 21 000 × g for 5 min at 4°C.
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عرض المزيد(B–F) Comparison of synthesis rates in different growth media and for additional species for the same pathways as Figure 1 (same display convention), with the addition that points marked by crosses had < 128 reads mapped to the corresponding gene for at least one of the two conditions shown in the pairwise comparison.
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عرض المزيدThis invention relates to enzymes and methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable …
عرض المزيدDynabeads Protein A are uniform, 2.8 µm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface. Dynabeads Protein A provide a superior alternative to Sepharose resin or agarose resin for immunoprecipitation (IP), and both manual and automated protocols are available.
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عرض المزيدEndogenous ACE2 is a receptor for SARS-CoV-2 and a recombinant soluble ACE2 protein can inhibit SARS-CoV-2 infection acting as a decoy. Here the authors show that B38-CAP, an ACE2-like enzyme but ...
عرض المزيدABOUT US Shandong Shengye Grinding Bal Co, Ltd is a professional factory specialize in the production of forged / hot rolled grinding balls, alloy grinding steel rods for more than 10 years, annual output is 100,000 tons. we have 10 worlds advanced forging and automatic rolling production lines that can meet customers' complex demands. ShengYe has passed …
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عرض المزيدThis invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors.
عرض المزيدSalmonella contamination of livestock feed is a serious veterinary and public health issue. In this study we used a systematic review to assess the prevalence and characterization of Salmonella isolates detected in raw feed components, feed milling equipment and finished feed from 97 studies published from 1955 to 2020 across seven global regions. . Eighty-five studies …
عرض المزيدA single-cell suspension was obtained by grinding the tissues using 1 ml syringe plunger and passed through a 70-μm strainer. Mononuclear cells were obtained by Percoll gradient (30/70%, Sigma, St. Louis, USA) centrifugation at 500 g for 30 min at room temperature (20–25°C) collected from the interphase, washed and used for further analysis.
عرض المزيدIntroduction. Endogenous or adoptively transferred cytotoxic T cells are essential mediators of antitumor immunity (Waldman et al., 2020).Persistent antigen exposure causes T cells to progressively differentiate into an exhausted state characterized by the hierarchical loss of effector functions and proliferation ability, as well as distinct transcriptional, epigenetic, and …
عرض المزيدThe eukaryotic replisome is a critical determinant of genome integrity with a complex structure that remains poorly characterized. A central unresolved issue is how the Cdc45-MCM-GINS helicase is linked to DNA polymerase epsilon, which synthesizes the leading strand at replication forks and is an important focus of regulation.
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